Rekombinanter mScarlet Antikörper (Alexa Fluor 647)

Produktnummer ABIN6953250

Dieses Alpaca Monoklonal-Antikörper erkennt spezifisch in IF, FACS und ICC. Er zeigt eine Reaktivität gegenüber Dictyosoma.

Kurzübersicht für Rekombinanter mScarlet Antikörper (Alexa Fluor 647) (ABIN6953250)

Target: mScarlet

Antikörpertyp: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reaktivität: Dictyosoma

Wirt: Alpaca

Klonalität: Monoklonal

Konjugat: Alexa Fluor 647

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Klon: 2B12

Produktdetails

Expressionssystem: E.coli

Verwendungszweck: Camelid sdAb anti-mScarlet-i conjugated with Alexa647, Clone 2B12

Spezifität: Recognizes mScarlet-I and most fluorescent proteins from Dictiosoma sp. and most common RFP derivatives

Kreuzreaktivität (Details): Does not cross-react with GFP, CFP, YFP and mTagBFP or their most common derivatives.

Produktmerkmale: A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.

Aufreinigung: Produced in: E.coli

Labeling Ratio: Two site-specifically conjugated fluorophores per sdAb.

Anwendungsinformationen

Applikationshinweise: Recommended dilution 1:500

Kommentare:

Two site-specifically coupled fluorophores per molecule. The reagent can therefore simultaneously target two fluorophores to your protein of interest, which results in enhanced image brightness. Owing to the small size of the sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this sdAb can thus improve the localization accuracy by 10-15 nm. Both features - enhanced brightness and precise fluorophore placement - renders this product superior tools for all microscopy techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable.

Konzentration: 5 μM

Buffer: lyophilized from PBS pH7.4 with 2% BSA (US-Origin)

Handhabung: Protect from light!

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Up to 3 months store at -20 °C. Up to 12 months store at -80 °C or below

Haltbarkeit: 12 months

Antigendetails

Target: mScarlet